Spectrin beta III (SPTBN2) [SPTBN2/3142R]



Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Product Properties & Targets

Species Reactivity
Cellular Localization
Gene Name
Mol. Weight of Antigen
Recombinant fragment (around aa356-475) of human SPTBN2 protein (exact sequence is proprietary)

Database Links

Entrez Gene ID

Additional Information

Human Gene Symbol
Chromosome Location

Known Applications & Suggested Dilutions

  • ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
  • Western Blot (1-2ug/ml)
  • Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml)
  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Michael C. Stankewich, et al. PNAS, 1998. 95 (24) 14158-14163.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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