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Spectrin beta III (SPTBN2) Antibody [SPTBN2/1583]

In Stock
Catalog Number Formulation Size Price
6712-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6712-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6712-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-2ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell cortex, Cytoplasm, Cytoskeleton
Gene Name
Positive Control
HeLa cells. Pancreas or Liver., PANC-1
Immunogen
Recombinant fragment (around aa356-475) of human SPTBN2 protein (exact sequence is proprietary)
Alternate Names
Spectrin beta chain, non-erythrocytic 2, Beta-III spectrin, Spinocerebellar ataxia 5 protein, Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
SPTBN2/1583
Chromosome Location
11q13.2
Mol. Weight of Antigen
246kDa

Functions

  • Probably plays an important role in neuronal membrane skeleton.

Key References

  • Speicher, D.W. 1986. The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of proteins. J. Cell. Biochem. 30: 245-258.
  • Coelman, T.R., et al. J.S. 1989. Functional diversity among spectrin isoforms. Cell Motil. Cytoskeleton 12: 225-247.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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