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Home >> Antibodies >> Spectrin beta III (SPTBN2)

Spectrin beta III (SPTBN2)

Mouse Monoclonal Antibody [Clone SPTBN2/1583]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1583).

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Western Blot Analysis of human HeLa cell lysate using Spectrin beta III Monoclonal Antibody (SPTBN2/1583).

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Confocal Immunofluorescence of HeLa cells. Spectrin beta III Monoclonal Antibody (SPTBN2/1583) labeled with CF488 (Green). Reddot is used to label the nuclei.

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SDS-PAGE Analysis Purified Spectrin beta III Monoclonal Antibody (SPTBN2/1583) labeled with CF488 (Green). Confirmation of Integrity and Purity of Antibody.

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Flow Cytometric Analysis of HeLa cells. Spectrin beta III Monoclonal Antibody (SPTBN2/1583) labeled with CF488 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Western Blot Analysis of human PANC-1 cell lysate using Spectrin beta III Monoclonal Antibody (SPTBN2/1583).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1583).

Product Details

Synonyms

Beta III spectrin; SCA5; Spectrin beta chain brain 2; Spectrin beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; Spinocerebellar ataxia 5; SPTBN2

Positive Control

PANC-1, HeLa cells. Pancreas or Liver.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
(,Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa356-475) of human SPTBN2 protein (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

246kDa

Specificity & Comments

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Speicher, D.W. 1986. The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of proteins. J. Cell. Biochem. 30: 245-258.
  2. Coelman, T.R., et al. J.S. 1989. Functional diversity among spectrin isoforms. Cell Motil. Cytoskeleton 12: 225-247.

Bioinformatics

  •  Entrez Gene ID: 6712
  •  Gene Symbol: SPTBN2
  •  SwissProt: O15020
  •  Chromosome Location: 11q13.2
  •  Unigene: 26915
NBT01583