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Home >> Antibodies >> Spectrin beta I (SPTB)

Spectrin beta I (SPTB)

Mouse Monoclonal Antibody [Clone RG/26]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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SDS-PAGE Analysis Purified Spectrin beta III Mouse Monoclonal Antibody (RG/26). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

beta-I spectrin; EL3; erythrocyte; HS2; SPTB1; HSPTB1; membrane cytoskeletal protein; spectrin beta (erythrocytic)

Positive Control

Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Known Applications & Suggested Dilutions

ELISA, Western Blot, IF, IHC-P         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Red cell membranes

Cellular Localization

Cell Surface

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

246kDa

Specificity & Comments

Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sachanonta et al. 2008. Asian Pac J Allergy Immunol. 26(1):37-45. PMID: 18595528.

Bioinformatics

  •  Entrez Gene ID: 6710
  •  Gene Symbol: SPTB
  •  SwissProt: O15020
  •  Chromosome Location: 14q23.3
  •  Unigene: 26915
NBT03057