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Home >> Antibodies >> Sarcomeric Actinin Alpha 2 / ACTN2

Sarcomeric Actinin Alpha 2 / ACTN2

Mouse Monoclonal Antibody [Clone ACTN2/3294]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human skeletal muscle stained with Sarcomeric Actinin Alpha 2 Mouse Monoclonal Antibody (ACTN2/3294).

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Formalin-fixed, paraffin-embedded human skeletal muscle stained with Sarcomeric Actinin Alpha 2 Mouse Monoclonal Antibody (ACTN2/3294).

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Formalin-fixed, paraffin-embedded human cardiac muscle stained with Sarcomeric Actinin Alpha 2 Mouse Monoclonal Antibody (ACTN2/3294).

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SDS-PAGE Analysis Purified ACTN2 Mouse Monoclonal Antibody (ACTN2/3294). Confirmation of Purity and Integrity of Antibody.

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Western Blot of human skeletal muscle tissue lysates using Sarcomeric Actinin Alpha 2 Mouse Monoclonal Antibody (ACTN2/3294).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Sarcomeric Actinin Alpha 2 Mouse Monoclonal Antibody (ACTN2/3294).

Product Details

Synonyms

Actin binding protein; Alpha actinin skeletal muscle, isoform 2; CMD1AA; F-actin cross-linking protein

Positive Control

Skeletal or Cardiac Muscle.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A recombinant fragment (aa557-692) of human ACTN2 protein (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

103kDa

Specificity & Comments

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Baris, O., et al. Oncogene 24 (April 4, 2005): 4155.

Bioinformatics

  •  Entrez Gene ID: 88
  •  Gene Symbol: ACTN2
  •  SwissProt: P35609
  •  Chromosome Location: 1q43
  •  Unigene: 498178
NBT03294