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Sarcomeric Actinin Alpha 2 / ACTN2 Antibody [ACTN2/3294]

In Stock
Catalog Number Formulation Size Price
88-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
88-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
88-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm, Myofibril, Sarcomere, Z line
Gene Name
Positive Control
Skeletal or Cardiac Muscle.
Immunogen
A recombinant fragment (aa557-692) of human ACTN2 protein (exact sequence is proprietary)
Alternate Names
Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, Actin binding protein; Alpha actinin skeletal muscle, isoform 2; CMD1AA; F-actin cross-linking protein

Database Links

Entrez Gene ID
88
SwissProt

Additional Information

Clone
ACTN2/3294
Chromosome Location
1q43
Mol. Weight of Antigen
103kDa

Functions

  • F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Key References

  • Baris, O., et al. Oncogene 24 (April 4, 2005): 4155.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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