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Home >> Antibodies >> S100A2 / S100 Calcium Binding Protein A2

S100A2 / S100 Calcium Binding Protein A2

Mouse Monoclonal Antibody [Clone CPTC-S100A2-2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Skin stained with S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2).

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Formalin-fixed, paraffin-embedded human Placenta stained with S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2).

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Immunofluorescence Analysis of A549 cells labeling S100A2 with S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

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Flow Cytometric Analysis of trypsinised HeLa cells. S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Western Blot Analysis of A431 cell lysate using S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2)

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Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A2 Mouse Monoclonal Antibody (CPTC-S100A2-2).

Product Details

Synonyms

CAN19; MGC111539; S100L

Positive Control

HeLa. A549, HACAT, BxPC3 and A431 cell lysates; Skin and squamous cell cervical carcinoma.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human S100A2 protein

Cellular Localization

Nucleus and cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

11.12kDa

Specificity & Comments

S100A2 belongs to the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100A2 may function as a modulator against excess calcium accumulation in normal human mammary epithelial cells and also have a role in suppressing tumor cell growth. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer. Cytoplasmic overexpression may also be of prognostic significance when observed in oral cancer patients, and the S100A2 has also been identified as significantly down-regulated in gastric cancer.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wessels D, et al. PLoS ONE 12(3): e0173400.

Bioinformatics

  •  Entrez Gene ID: 6273
  •  Gene Symbol: S100A2
  •  SwissProt: P29034
  •  Chromosome Location: 1q21.3
  •  Unigene: 516484
NBT02591