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Home >> Antibodies >> Renal Cell Carcinoma (Carbonic Anhydrase IX)

Renal Cell Carcinoma (Carbonic Anhydrase IX)

Mouse Monoclonal Antibody [Clone CA9/3406]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human kidney stained with CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Formalin-fixed, paraffin-embedded human kidney stained with CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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SDS-PAGE Analysis Purified CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406). Confirmation of Integrity and Purity of Antibody.

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Western blot analysis of HEK293 cell lysate using RCC Mouse Monoclonal Antibody (CA9/3406).

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Western blot analysis of U-87 cell lysate using RCC Mouse Monoclonal Antibody (CA9/3406).

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Flow Cytometric Analysis of PFA-fixed U87MG cells using CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406).

Product Details

Synonyms

CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

Positive Control

Normal kidney or renal cell carcinoma (IHC).

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human CA9 protein fragment (around aa 314-410) (exact sequence is proprietary)

Cellular Localization

Cell Surface. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse IgG2b, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yoshida SO, et. al. Cancer Research, 1989, 49(7):1802-9.

Bioinformatics

  •  Entrez Gene ID: 768
  •  Gene Symbol: CA9
  •  SwissProt: Q16790
  •  Chromosome Location: 9p13.3
  •  Unigene: 63287