Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Recombinant Renal Cell Carcinoma (Carbonic Anhydrase IX) Antibody [CA9/9621R]

In Stock
Catalog Number Formulation Size Price
768-RBM17-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
768-RBM17-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
768-RBM17-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman’s capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Product Properties & Targets

Host
Rabbit
Species Reactivity
Research Areas
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Cell projection, Microvillus membrane, Nucleolus, Nucleus
Gene Name
CA9
Positive Control
HeLa cells. Normal kidney or renal cell carcinoma (IHC).
Immunogen
Extracellular N-terminal proteoglycan-like region. Epitope is between aa80-100.
Alternate Names
Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

Database Links

Entrez Gene ID
768
SwissProt

Additional Information

Clone
CA9/9621R
Chromosome Location
9p13.3
Mol. Weight of Antigen
55kDa

Functions

  • Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

Key References

  • Yoshida SO, et. al. Cancer Research, 1989, 49(7):1802-9.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant Renal Cell Carcinoma (Carbonic Anhydrase IX) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK