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Home >> Antibodies >> Renal Cell Carcinoma (Carbonic Anhydrase IX)

Renal Cell Carcinoma (Carbonic Anhydrase IX)

Recombinant Rabbit Monoclonal Antibody [Clone CA9/2993R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with RCC Rabbit Recombinant Monoclonal Antibody (CA9/2993R).

img

SDS-PAGE Analysis Purified RCC Rabbit Recombinant Monoclonal Antibody (CA9/2993R). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

CA-IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

Positive Control

Normal kidney or renal cell carcinoma (IHC).

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CA9 protein

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human. Horse.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

55kDa

Specificity & Comments

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yoshida SO, et. al. Cancer Research, 1989, 49(7):1802-9.

Bioinformatics

  •  Entrez Gene ID: 768
  •  Gene Symbol: CA9
  •  SwissProt: Q16790
  •  Chromosome Location: 9p13.3
  •  Unigene: 63287
NBT02993