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Home >> Antibodies >> Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker)

Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker)

Mouse Monoclonal Antibody [Clone 2A4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Monoclonal Antibody (2A4).

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Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Monoclonal Antibody (2A4).

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Western Blot Analysis of HeLa cell lysate using Podocalyxin Monoclonal Antibody (2A4).

Product Details

Synonyms

PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1

Positive Control

HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Cellular Localization

Cell surface and Cytoplasmic

Species Reactivity

Human. Rabbit. Rat.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

165-170kDa

Specificity & Comments

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kershaw DB et. al. J Biol Chem 272:15708-15714 (1997).
  2. Sassetti C et. al. J Exp Med187:1965-1975. 3. Nielsen JS et. al. J Am Soc Nephrol 20:1669-1676 (2009).

Bioinformatics

  •  Entrez Gene ID: 5420
  •  Gene Symbol: PODXL
  •  SwissProt: O00592
  •  Chromosome Location: 7q32.3
  •  Unigene: 732423
NBT00483