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Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Antibody [2A4]

In Stock
Catalog Number Formulation Size Price
5420-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5420-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5420-MSM3-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Monoclonal Antibody (2A4).

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Monoclonal Antibody (2A4).

Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Monoclonal Antibody (2A4).

Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Monoclonal Antibody (2A4).

Western Blot Analysis of HeLa cell lysate using Podocalyxin Monoclonal Antibody (2A4).

Western Blot Analysis of HeLa cell lysate using Podocalyxin Monoclonal Antibody (2A4).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Rabbit, Rat
Research Areas
Endothelial Cell Marker, Stem Cell Differentiation
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Apical cell membrane, Cell projection, Filopodium, Lamellipodium, Membrane, Membrane raft, Microvillus, Ruffle
Gene Name
PODXL
Positive Control
Angiosarcoma, Breast, HeLa, Jurkat cells, Kidney., Liver, Pancreatic, prostate, Raji
Immunogen
A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin
Alternate Names
Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1

Database Links

Entrez Gene ID
5420
SwissProt
O00592

Additional Information

Clone
2A4
Chromosome Location
7q32.3
Mol. Weight of Antigen
165-170kDa

Related Antibodies

View All PODXL Antibodies

Functions

  • Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

Key References

  • Kershaw DB et. al. J Biol Chem 272:15708-15714 (1997).
  • Sassetti C et. al. J Exp Med187:1965-1975. 3. Nielsen JS et. al. J Am Soc Nephrol 20:1669-1676 (2009).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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