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Home >> Antibodies >> PDZ and LIM Domain 1

PDZ and LIM Domain 1

Mouse Monoclonal Antibody [Clone CPTC-PDLIM1-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with PDZ and LIM Domain 1 Mouse Monoclonal Antibody (PDLIM-1).

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SDS-PAGE Analysis Purified PDZ and LIM Domain 1 Mouse Monoclonal Antibody (PDLIM-1). Confirmation of Purity and Integrity of Antibody

Product Details

Synonyms

C terminal LIM domain protein 1; carboxy terminal LIM domain protein 1; CLIM1; CLP36; Elfin; Enigma antibody; Enigma homolog antibody; hCLIM1; PDLIM1; PDZ and LIM domain 1

Positive Control

HepG2 cells and extracts from Jurkat. Human heart, colon or spleen.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length PDLIM1 protein

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

36kDa

Specificity & Comments

CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88% sequence similarity with its rat counterpart, suggesting a conserved function between species.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bauer, K., et al. 2000. Blood 96: 4236-4245

Bioinformatics

  •  Entrez Gene ID: 9124
  •  Gene Symbol: PDLIM1
  •  SwissProt: O00151
  •  Chromosome Location: 10q23.33
  •  Unigene: 368525
NBT02272