Western blot analysis of human heart tissue lysate using N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/6857R).
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/6857R). HIER: Tris/EDTA, pH9.0, 45min. 2░: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/6857R).
SDS-PAGE Analysis Purified NCAD Recombinant Rabbit Monoclonal Antibody (CDH2/6857R). Confirmation of Purity and Integrity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. áStudies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.