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Home >> Antibodies >> Myeloid-Associated Differentiation Marker (MYADM)

Myeloid-Associated Differentiation Marker (MYADM)

Mouse Monoclonal Antibody [Clone MYADM/972]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with MYDAM Monoclonal Antibody (MYADM/972).

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SDS-PAGE Analysis Purified MYDAM Monoclonal Antibody (MYADM/972). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

MYADM; myeloid associated differentiation marker; Myeloid upregulated protein; Protein SB135

Positive Control

HL60 cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human MYADM protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Macaque Monkey.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Unknown

Specificity & Comments

Recognizes a myeloid associated differentiation antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of human and monkey myeloid cells. This MAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Membrane protein hMYADM preferentially expressed in myeloid cells is up-regulated during differentiation of stem cells and myeloid leukemia cells. Wang Q, et al. Life Sci, 2007 Jan 9. PMID 17097684
  2. Cloning of human myeloid-associated differentiation marker (MYADM) gene whose expression was up-regulated in NB4 cells induced by all-trans retinoic acid. Cui W, et al. Mol Biol Rep, 2001. PMID 12075932
  3. MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration. Aranda JF, et al. Mol Biol Cell, 2011 Apr 15. PMID 21325632
  4. MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1 expression. Aranda JF, et al. Mol Biol Cell, 2013 Feb. PMID 23264465
  5. Isolation of MYADM, a novel hematopoietic-associated marker gene expressed in multi-potent progenitor cells and up-regulated during myeloid differentiation. Pettersson M, et al. J Leukoc Biol, 2000 Mar. PMID 10733104

Bioinformatics

  •  Entrez Gene ID: 91663
  •  Gene Symbol: MYADM
  •  SwissProt: Q96S97
  •  Chromosome Location: 19q13.42
  •  Unigene: 380906
NBT00972