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Home >> Antibodies >> MXI1

MXI1

Mouse Monoclonal Antibody [Clone PCRP-MXI1-1A3]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. MXI1 Mouse Monoclonal Antibody (PCRP-MXI1-1A3) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence analysis of PFA-fixed HeLa cells. MXI1 Mouse Monoclonal Antibody (PCRP-MXI1-1A3)

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SDS-PAGE Analysis Purified MXI1 Mouse Monoclonal Antibody (PCRP-MXI1-1A3). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using MXI1 Mouse Monoclonal Antibody (PCRP-MXI1-1A3).

Product Details

Synonyms

bHLHc11; Class C basic helix-loop-helix protein 11; MAX dimerization protein 2 (MAD2); MAX interacting protein 1; Max interactor 1; Max related transcription factor; MXD2; MXI; MXI1 protein

Positive Control

HeLa or K562 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MXI1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

26/22/33/21 kDa (isoforms)

Specificity & Comments

It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (also designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad- binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-1492.
  2. Ayer, D.E., Kretzner, L. and Eisenman, R.N. 1993. Cell 72: 211-222.

Bioinformatics