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MXI1 Antibody [PCRP-MXI1-1A3]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
4601-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4601-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4601-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-2ug/ml
Western Blot (WB)
2-4ug/ml

Summary

It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (also designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad- binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b /
Cellular Localization
Nucleus
Gene Name
Positive Control
HeLa or K562 cells.
Immunogen
Recombinant full-length human MXI1 protein
Alternate Names
Max-interacting protein 1, Class C basic helix-loop-helix protein 11, bHLHc11; Class C basic helix-loop-helix protein 11; MAX dimerization protein 2 (MAD2); MAX interacting protein 1; Max interactor 1; Max related transcription factor; MXD2; MXI; MXI1 protein

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-MXI1-1A3
Chromosome Location
10q25.2
Mol. Weight of Antigen
26/22/33/21 kDa (isoforms)

Functions

  • Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX.

Key References

  • Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-1492.
  • Ayer, D.E., Kretzner, L. and Eisenman, R.N. 1993. Cell 72: 211-222.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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