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Home >> Antibodies >> MSH6 (DNA Mismatch Repair Protein)

MSH6 (DNA Mismatch Repair Protein)

Recombinant Rabbit Monoclonal Antibody [Clone MSH6/4592R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human colon stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/4592R).

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Western blot analysis of HCT-116 cell lysate using MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/4592).

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SDS-PAGE Analysis Purified MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/4592R). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human colon stained with MSH6 Recombinant Rabbit Monoclonal Antibody (MSH6/4592R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS (E. coli) homolog 6; MutS alpha 160kDa subunit; MutS-alpha 160kDa subunit; p160; Sperm associated protein

Positive Control

HCT116, MCF-7 or HeLa cells. Human colon carcinoma or tonsil.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (For coating, order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determin

Immunogen

Recombinant fragment (around aa 374-540) of human MSH6 protein (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

163kDa

Specificity & Comments

The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Peltomaki, P., et al. 1993. Genetic mapping of a locus predisposing to human colorectal cancer. Science 260: 810-812.

Bioinformatics

  •  Entrez Gene ID: 2956
  •  Gene Symbol: MSH6
  •  SwissProt: P52701
  •  Chromosome Location: 2p16.3
  •  Unigene: 445052