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Home >> Antibodies >> MAML3 (Mastermind Like Transcriptional Coactivator 3)

MAML3 (Mastermind Like Transcriptional Coactivator 3)

Mouse Monoclonal Antibody [Clone MAML3/1303]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Pancreas stained with MAML3 Monoclonal Antibody (MAML3/1303).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MAML3 Monoclonal Antibody (MAML3/1303).

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Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with MAML3 Monoclonal Antibody (MAML3/1303).

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Formalin-fixed, paraffin-embedded human Placenta stained with MAML3 Monoclonal Antibody (MAML3/1303).

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SDS-PAGE Analysis Purified MAML3 Monoclonal Antibody (MAML3/1303). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CAGH3; ERDA3; GDN; MAML3; Mastermind-like protein 3; TNRC3

Positive Control

MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human MAML3 protein fragment

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

150-170kDa

Specificity & Comments

MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wu, L., Sun, T., Kobayashi, K., Gao, P. and Griffin, J.D. 2002. Identification of a family of mastermind-like transcriptional co-activators for mammalian Notch receptors. Mol. Cell. Biol. 22: 7688-7700

Bioinformatics

  •  Entrez Gene ID: 55534
  •  Gene Symbol: MAML3
  •  SwissProt: Q96JK9
  •  Chromosome Location: 4q28
  •  Unigene: 586165
NBT01303