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Home >> Antibodies >> MAML2 (Mastermind Like Transcriptional Coactivator 2)

MAML2 (Mastermind Like Transcriptional Coactivator 2)

Mouse Monoclonal Antibody [Clone MAML2/1302]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Pancreas stained with MAML2 Monoclonal Antibody (MAML2/1302).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MAML2 Monoclonal Antibody (MAML2/1302).

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Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with MAML2 Monoclonal Antibody (MAML2/1302).

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Formalin-fixed, paraffin-embedded human Placenta stained with MAML2 Monoclonal Antibody (MAML2/1302).

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SDS-PAGE Analysis Purified MAML2 Monoclonal Antibody (MAML2/1302). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Mam-2; MAML2; Mastermind-like protein 2

Positive Control

MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human MAML2 fragment

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

125kDa

Specificity & Comments

MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wu, L., Sun, T., Kobayashi, K., Gao, P. and Griffin, J.D. 2002. Identification of a family of mastermind-like transcriptional co-activators for mammalian Notch receptors. Mol. Cell. Biol. 22: 7688-7700

Bioinformatics

  •  Entrez Gene ID: 84441
  •  Gene Symbol: MAML2
  •  SwissProt: Q8IZL2
  •  Chromosome Location: 11q21
  •  Unigene: 428214
NBT01302