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Home >> Antibodies >> Interferon regulatory factor 9 / IRF-9

Interferon regulatory factor 9 / IRF-9

Mouse Monoclonal Antibody [Clone PCRP-IRF9-2F8]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis. Purified IRF9 Mouse Monoclonal Antibody (PCRP-IRF9-2F8). Confirmation of Purity and Integrity of Antibody.

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Flow cytometric analysis of PFA-fixed HeLa cells. IRF9 Mouse Monoclonal Antibody (PCRP-IRF9-2F8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescent analysis of PFA-fixed HeLa cells. IRF9 Mouse Monoclonal Antibody (PCRP-IRF9-2F8) followed by goat anti-mouse IgG-CF488 (green); counterstain (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using IRF9 Mouse Monoclonal Antibody (PCRP-IRF9-2F8).

Product Details

Synonyms

IFN alpha responsive transcription factor subunit; Interferon regulatory factor 9; Interferon-stimulated gene factor 3 gamma; interferon-stimulated transcription factor 3, gamma 48kDa; IRF-9; ISGF-3 gamma; ISGF3 p48 subunit; ISGF3G; p48; Transcriptional regulator ISGF3 subunit gamma

Positive Control

MCF-7, Jurkat or K562 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human IRF9 protein

Cellular Localization

Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG2a

Mol. Weight of Antigen

43.34kDa

Specificity & Comments

Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.
  2. Nucleic Acids Res. 38:D142-D148 (2010).

Bioinformatics

  •  Entrez Gene ID: 10379
  •  Gene Symbol: IRF9
  •  SwissProt: Q00978
  •  Chromosome Location: 14q12
  •  Unigene: 1706