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Interferon regulatory factor 9 / IRF-9 Antibody [PCRP-IRF9-2F8]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
10379-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
10379-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
10379-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-2ug/ml

Summary

Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2a /
Cellular Localization
Cytoplasm, Nucleus
Gene Name
Positive Control
Jurkat or K562 cells., MCF-7
Immunogen
Recombinant full-length human IRF9 protein
Alternate Names
Interferon regulatory factor 9, IFN-alpha-responsive transcription factor subunit, ISGF3 p48 subunit, Interferon-stimulated gene factor 3 gamma, Transcriptional regulator ISGF3 subunit gamma, IFN alpha responsive transcription factor subunit; Interferon regulatory factor 9; Interferon-stimulated gene factor 3 gamma; interferon-stimulated transcription factor 3, gamma 48kDa; IRF-9; ISGF-3 gamma; ISGF3 p48 subunit; ISGF3G; p48; Transcriptional regulator ISGF3 subunit gamma

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-IRF9-2F8
Chromosome Location
14q12
Mol. Weight of Antigen
43.34kDa

Functions

  • Transcription factor that plays an essential role in anti-viral immunity. It mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Key References

  • Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.
  • Nucleic Acids Res. 38:D142-D148 (2010).

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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