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Home >> Antibodies >> Integrase interactor 1 (INI-1) / SNF5 / SMARCB1

Integrase interactor 1 (INI-1) / SNF5 / SMARCB1

Mouse Monoclonal Antibody [Clone SMARCB1/3984]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human testis stained with INI-1Mouse Monoclonal Antibody (SMARCB1/3984) at 2ug/ml. Inset: PBS instead of primary antibody, secondary negative control.

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Formalin-fixed, paraffin-embedded human epithelioid sarcoma stained with INI-1 Mouse Monoclonal Antibody (SMARCB1/3984).

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Formalin-fixed, paraffin-embedded human epithelioid sarcoma stained with INI-1 Mouse Monoclonal Antibody (SMARCB1/3984).

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SDS-PAGE Analysis Purified INI-1 Mouse Monoclonal Antibody (SMARCB1/3984). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using INI-1-Monospecific Mouse Monoclonal Antibody (SMARCB1/3984).

Product Details

Synonyms

BRG1-associated factor 47 (BAF47); hSNF5; Malignant rhabdoid tumor suppressor; RDT; RTPS1; Sfh1p; Snr1; Sucrose nonfermenting yeast homolog like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; SWI10; Transcription factor TYE4; Transcription regulatory protein SNF5

Positive Control

Human brain, astrocytoma or kidney.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 65-184) of human SMARCB1 protein (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

47kDa

Specificity & Comments

The INI-1 gene, which encodes a functionally uncharacterized protein component of the hSWI/SNF chromatin remodeling complex, is often mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI-1, that differ by the variable inclusion of amino acids, potentially are produced by differential RNA splicing. The morphology of MRTs can present challenges in differential diagnosis. The overall survival of MRTs relative to its potential mimics (medulloblastoma, supratentorial primitive neuroectodermal tumors (sPNETs) is quite low, and thus differentiation from these other tumors is desirable. Lack of nuclear labeling by anti-INI-1 is characteristic of MRT. The majority of medulloblastomas and sPNETs are labeled by anti-INI-1. MRTs also originate from the kidney and soft tissues.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bourdeaut F, et al. J Pathol. 2007; 211:323-30.
  2. Fowler DJ, et al. Fetal PediatrPathol. 2006; 25:159-68.

Bioinformatics

  •  Entrez Gene ID: 6598
  •  Gene Symbol: SMARCB1
  •  SwissProt: Q12824
  •  Chromosome Location: 22q11.23
  •  Unigene: 534350