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Recombinant Integrase interactor 1 (INI-1) / SNF5 / SMARCB1 Antibody [rSMARCB1/4588]

In Stock
Catalog Number Formulation Size Price
6598-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6598-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6598-MSM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human epithelioid sarcoma stained with INI-1 Recombinant Mouse Monoclonal Antibody (rSMARCB1/4588). Note loss of nuclear expression in tumor cells and retained positivity in normal cells.

Formalin-fixed, paraffin-embedded human epithelioid sarcoma stained with INI-1 Recombinant Mouse Monoclonal Antibody (rSMARCB1/4588). Note loss of nuclear expression in tumor cells and retained positivity in normal cells.

Formalin-fixed, paraffin-embedded human Ewing sarcoma stained with INI-1 Recombinant Mouse Monoclonal Antibody (rSMARCB1/4588). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human Ewing sarcoma stained with INI-1 Recombinant Mouse Monoclonal Antibody (rSMARCB1/4588). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The INI-1 gene, which encodes a functionally uncharacterized protein component of the hSWI/SNF chromatin remodeling complex, is often mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI-1, that differ by the variable inclusion of amino acids, potentially are produced by differential RNA splicing. The morphology of MRTs can present challenges in differential diagnosis. The overall survival of MRTs relative to its potential mimics (medulloblastoma, supratentorial primitive neuroectodermal tumors (sPNETs) is quite low, and thus differentiation from these other tumors is desirable. Lack of nuclear labeling by anti-INI-1 is characteristic of MRT. The majority of medulloblastomas and sPNETs are labeled by anti-INI-1. MRTs also originate from the kidney and soft tissues.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Nuclear Marker, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
SMARCB1
Positive Control
kidney or astrocytoma., Ubiquitous nuclear expression. Human brain
Immunogen
Recombinant fragment of mouse BAF47 protein (around aa257-359) (exact sequence is proprietary)
Alternate Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, BRG1-associated factor 47, Integrase interactor 1 protein, SNF5 homolog, BRG1-associated factor 47 (BAF47); hSNF5; Malignant rhabdoid tumor suppressor; RDT; RTPS1; Sfh1p; Snr1; Sucrose nonfermenting yeast homolog like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; SWI10; Transcription factor TYE4; Transcription regulatory protein SNF5

Database Links

Entrez Gene ID
6598
SwissProt
Q12824

Additional Information

Clone
rSMARCB1/4588
Chromosome Location
22q11.23
Mol. Weight of Antigen
47kDa

Related Antibodies

View All SMARCB1 Antibodies

Functions

  • Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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