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Home >> Antibodies >> Integrase interactor 1 (INI-1) / SNF5 / SMARCB1

Integrase interactor 1 (INI-1) / SNF5 / SMARCB1

Recombinant Mouse Monoclonal Antibody [Clone rSMARCB1/4588]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human epithelioid sarcoma stained with INI-1 Recombinant Mouse Monoclonal Antibody (rSMARCB1/4588). Note loss of nuclear expression in tumor cells and retained positivity in normal cells.

img

Formalin-fixed, paraffin-embedded human Ewing sarcoma stained with INI-1 Recombinant Mouse Monoclonal Antibody (rSMARCB1/4588). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

BRG1-associated factor 47 (BAF47); hSNF5; Malignant rhabdoid tumor suppressor; RDT; RTPS1; Sfh1p; Snr1; Sucrose nonfermenting yeast homolog like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; SWI10; Transcription factor TYE4; Transcription regulatory protein SNF5

Positive Control

Ubiquitous nuclear expression. Human brain, kidney or astrocytoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of mouse BAF47 protein (around aa257-359) (exact sequence is proprietary)

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

47kDa

Specificity & Comments

The INI-1 gene, which encodes a functionally uncharacterized protein component of the hSWI/SNF chromatin remodeling complex, is often mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI-1, that differ by the variable inclusion of amino acids, potentially are produced by differential RNA splicing. The morphology of MRTs can present challenges in differential diagnosis. The overall survival of MRTs relative to its potential mimics (medulloblastoma, supratentorial primitive neuroectodermal tumors (sPNETs) is quite low, and thus differentiation from these other tumors is desirable. Lack of nuclear labeling by anti-INI-1 is characteristic of MRT. The majority of medulloblastomas and sPNETs are labeled by anti-INI-1. MRTs also originate from the kidney and soft tissues.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Bioinformatics

  •  Entrez Gene ID: 6598
  •  Gene Symbol: SMARCB1
  •  SwissProt: Q12824
  •  Chromosome Location: 22q11.23
  •  Unigene: 534350