Home >> Antibodies >> HSP27 (Heat Shock Protein 27)

HSP27 (Heat Shock Protein 27)

Mouse Monoclonal Antibody [Clone HSPB1/774]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Monoclonal Antibody (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with HSP27 MAb (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

SDS-PAGE Analysis Purified HSP27 MAb (HSPB1/774). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774).

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Monoclonal Antibody (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with HSP27 MAb (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

SDS-PAGE Analysis Purified HSP27 MAb (HSPB1/774). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774).

Product Details

Synonyms

Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

Positive Control

HeLa or MCF-7 cells, ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (0.25-0.5ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human HSPB1 protein

Cellular Localization

Predominantly cytoplasmic with some nuclear

Species Reactivity

Human. Chimpanzee. Sheep. Rat. Mouse. Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

27kDa

Specificity & Comments

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.