Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (HSPB1/774)
Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HSP27 Monoclonal Antibody (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling HSP27 with HSP27 MAb (HSPB1/774) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
SDS-PAGE Analysis Purified HSP27 MAb (HSPB1/774). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of HeLa cell lysate using HSP27 MAb (HSPB1/774).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.