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Recombinant Heat Shock 27kDa Protein 1 Antibody [HSPB1/6490R]

In Stock
Catalog Number Formulation Size Price
3315-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3315-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3315-RBM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian 伪-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Product Properties & Targets

Host
Rabbit
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Cytoskeleton, Nucleus, Spindle
Gene Name
Positive Control
MCF-7 or HeLa cells. ~50% of breast carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR
Immunogen
Recombinant human full-length HSP27 protein
Alternate Names
Heat shock protein beta-1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Stress-responsive protein 27, Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
HSPB1/6490R
Chromosome Location
7q11.23
Mol. Weight of Antigen
27kDa

Functions

  • Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Key References

  • Georgopoulos, C. and Welch, W.J. 1993. Annu. Rev. Cell Biol. 9: 601-634

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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