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Home >> Antibodies >> HLA-Pan (MHC II)

HLA-Pan (MHC II)

Recombinant Mouse Monoclonal Antibody [Clone rHLA-Pan/3475]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475).

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Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475).

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Flow Cytometric Analysis of human Raji cells. HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Immunofluorescence Analysis of human Raji cells. HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475) followed by goat anti-mouse IgG-CF488 (green). Nuclear counterstain is RedDot.

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Western Blot Analysis of (1) Ramos cell and (2) human spleen tissue lysate using HLA-Pan Mouse Recombinant Monoclonal Antibody (rHLA-Pan/3475).

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SDS-PAGE Analysis Purified HLA-Pan Mouse Recombinant Monoclonal (rHLA-Pan/3475). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

HLA-DPB1; HLA-DQA1; HLA-DQB1; HLA-DRA; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5

Positive Control

Raji cells. Human tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cells

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

33-35kDa

Specificity & Comments

Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gatter et al. 1982. J Clin Pathol. 35(11):1253-67.
  2. Gatter et al. 1982. Semin Oncol. 9(4):517-25.

Bioinformatics