Home >> Antibodies >> Histone H1 (Pan Nuclear Marker)

Histone H1 (Pan Nuclear Marker)

Recombinant Rabbit Monoclonal Antibody [Clone HH1/1784R]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

Formalin-fixed, paraffin-embedded human Pancreas stained with Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R).

SDS-PAGE Analysis Purified Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R). Confirmation of Integrity and Purity of Antibody.

Flow Cytometric Analysis of PFA-fixed HeLa cells using Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescent staining of PFA-fixed HeLa cells using Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R) followed by goat anti-rabbit IgG-CF488 (green). Membrane labeled with Phalloidin.

Western Blot Analysis of human heart tissue lysate using Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R).

Formalin-fixed, paraffin-embedded human Pancreas stained with Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R).

SDS-PAGE Analysis Purified Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R). Confirmation of Integrity and Purity of Antibody.

Flow Cytometric Analysis of PFA-fixed HeLa cells using Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescent staining of PFA-fixed HeLa cells using Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R) followed by goat anti-rabbit IgG-CF488 (green). Membrane labeled with Phalloidin.

Western Blot Analysis of human heart tissue lysate using Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R).

Product Details

Synonyms

HANP1; His1; HisC; HIST1; HIST1H1A; HIST1H1B; HIST1H1C; HIST1H1D; HIST1H1E; HIST1H1T; Oocyte-specific histone H1; Testicular H1 histone

Positive Control

HeLa, A-431, LNCap or Jurkat cells. Human pancreas or breast carcinoma tissue. Human heart tissue lysate.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IF, IHC-P More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human Histone H1 protein

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

~30kDa

Specificity & Comments

Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.