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Home >> Antibodies >> Histone Deacetylase 1 (HDAC3)

Histone Deacetylase 1 (HDAC3)

Mouse Monoclonal Antibody [Clone PCRP-HDAC3-3C9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. HDAC3 Mouse Monoclonal Antibody (PCRP-HDAC3-3C9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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SDS-PAGE Analysis Purified HDAC3 Mouse Monoclonal Antibody (PCRP-HDAC3-3C9). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC3 Mouse Monoclonal Antibody (PCRP-HDAC3-3C9) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC3-Monospecific Mouse Monoclonal Antibody (PCRP-HDAC3-3C9).

Product Details

Synonyms

HD3; HDAC 3; HDAC3; HDAC3_HUMAN; Histone deacetylase 3; RPD3 2; RPD3; RPD3-2; SMAP45

Positive Control

HeLa or Jurkat cells.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IP, Flow Cyt, IF         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human HDAC3 protein

Cellular Localization

Nucleus. Nucleoplasm. Golgi apparatus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

49kDa

Specificity & Comments

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 8841
  •  Gene Symbol: HDAC3
  •  SwissProt: O15379
  •  Chromosome Location: 5q31.3
  •  Unigene: 519632