Flow cytometric analysis of PFA-fixed HeLa cells. HDAC3 Mouse Monoclonal Antibody (PCRP-HDAC3-3C9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
SDS-PAGE Analysis Purified HDAC3 Mouse Monoclonal Antibody (PCRP-HDAC3-3C9). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC3 Mouse Monoclonal Antibody (PCRP-HDAC3-3C9) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC3-Monospecific Mouse Monoclonal Antibody (PCRP-HDAC3-3C9).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.