These antibodies are validated on HuProt™ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE
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Flow cytometric analysis of PFA-fixed HeLa cells. HDAC3 Mouse Monoclonal Antibody (PCRP-HDAC3-3C9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
SDS-PAGE Analysis of Purified HDAC3 Mouse Monoclonal Antibody (PCRP-HDAC3-3C9). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC3 Mouse Monoclonal Antibody (PCRP-HDAC3-3C9) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC3-Monospecific Mouse Monoclonal Antibody (PCRP-HDAC3-3C9). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.
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