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Home >> Antibodies >> HER-4 / ERBB4

HER-4 / ERBB4

Mouse Monoclonal Antibody [Clone HFR-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified HER-4 / ERBB4 Mouse Monoclonal Antibody (HFR-1). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence Analysis of MCF-7 cells labeling HER-4 with HER-4 / ERBB4 Mouse Monoclonal Antibody (HFR-1) Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

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Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HER-4 / ERBB4 Mouse Monoclonal Antibody (HFR-1) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Avian erythroblastic leukemia viral oncogene homolog 4; ERBB4; HER4; human epidermal growth factor receptor 4; p180erbB4; Proto-oncogene-like protein c-ErbB-4

Positive Control

Breast, brain, heart or kidney. MCF-7 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human ERBB4 (HER4) protein fragment (around aa 1116-1269) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic, Cell Surface and Nuclear

Species Reactivity

Human. Mouse. Predicted to work with Rat, Chicken.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

180kDa (precursor); 80/120kDa (cleaved)

Specificity & Comments

The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full-length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. Its expression in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in studies which suggest ErbB4 expression is involved in both normal tissue development and carcinogenesis.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Plowman, G.D., et al. 1993. Proc. Natl. Acad. Sci. USA 90: 1746-1750

Bioinformatics

  •  Entrez Gene ID: 2066
  •  Gene Symbol: ERBB4
  •  SwissProt: Q15303
  •  Chromosome Location: 2q34
  •  Unigene: 390729
NBT02379