SDS-PAGE Analysis Purified HER-4 / ERBB4 Mouse Monoclonal Antibody (HFR-1). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of MCF-7 cells labeling HER-4 with HER-4 / ERBB4 Mouse Monoclonal Antibody (HFR-1) Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HER-4 / ERBB4 Mouse Monoclonal Antibody (HFR-1) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full-length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. Its expression in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in studies which suggest ErbB4 expression is involved in both normal tissue development and carcinogenesis.