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HER-4 / ERBB4 Antibody [HFR-1]

Additional information

Catalog Number: 2066-MSM5
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2066-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2066-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2066-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
c-erbB-4 / HER-4 Antibody in SDS-PAGE

c-erbB-4 / HER-4 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified HER-4 Mouse Monoclonal Antibody (HFR1). Confirmation of Purity and Integrity of Antibody

HER-4 / ERBB4 Antibody - Image 2

Immunofluorescence Analysis of MCF-7 cells labeling HER-4 with HER-4 / ERBB4 Mouse Monoclonal Antibody (HFR-1) Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

HER-4 / ERBB4 Antibody - Image 3

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using HER-4 / ERBB4 Mouse Monoclonal Antibody (HFR-1) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Flow
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human, Mouse
Clone
HFR-1
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cell membrane, Mitochondrion, Nucleus
Gene Name
ERBB4
Positive Control
Brain, Breast, heart or kidney. MCF-7 cells.
Immunogen
Recombinant human ERBB4 (HER4) protein fragment (around aa 1116-1269) (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.
Key References
  • Plowman, G.D., et al. 1993. Proc. Natl. Acad. Sci. USA 90: 1746-1750
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2066
SwissProt
Q15303
Chromosome Location
2q34
Mol. Weight of Antigen
180kDa (precursor); 80/120kDa (cleaved)
Alternate Names
Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4, Avian erythroblastic leukemia viral oncogene homolog 4; ERBB4; HER4; human epidermal growth factor receptor 4; p180erbB4; Proto-oncogene-like protein c-ErbB-4

Summary

The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full-length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. Its expression in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in studies which suggest ErbB4 expression is involved in both normal tissue development and carcinogenesis.

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