2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Heat Shock 27kDa Protein 1

Heat Shock 27kDa Protein 1

Mouse Monoclonal Antibody [Clone CPTC-HSPB1-2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

SDS-PAGE Analysis Purified HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2). Confirmation of Purity and Integrity of Antibody

img

Formalin-fixed, paraffin-embedded human prostate stained with HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2).

img

Flow cytometric analysis of PFA-fixed MCF-7 cells using HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

img

Western blot analysis of human liver tissue lysate using HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2).

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2).

Product Details

Synonyms

Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

Positive Control

MCF-7 or HeLa cells. ~50% of breast carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length protein

Cellular Localization

Predominantly cytoplasmic with some nuclear. It is cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

27kDa

Specificity & Comments

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Georgopoulos, C. and Welch, W.J. 1993. Annu. Rev. Cell Biol. 9: 601-634

Bioinformatics

  •  Entrez Gene ID: 3315
  •  Gene Symbol: HSPB1
  •  SwissProt: P04792
  •  Chromosome Location: 7q11.23
  •  Unigene: 520973