SDS-PAGE Analysis Purified HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2). Confirmation of Purity and Integrity of Antibody
Formalin-fixed, paraffin-embedded human prostate stained with HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2).
Immunofluorescence analysis of PFA-fixed MCF-7 cells using HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2) followed bygoat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot.
Flow cytometric analysis of PFA-fixed MCF-7 cells using HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Western blot analysis of human liver tissue lysate using HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2).
Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.