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Home >> Antibodies >> GRAMD4 (Transcription Factor)

GRAMD4 (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-GRAMD4-1A10]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. GRAMD4 Mouse Monoclonal Antibody (PCRP-GRAMD4-1A10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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SDS-PAGE Analysis. Purified GRAMD4 Mouse Monoclonal Antibody (PCRP-GRAMD4-1A10). Confirmation of Integrity and Purity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using GRAMD4 Mouse Monoclonal Antibody (PCRP-GRAMD4-1A10).

Product Details

Synonyms

Death-inducing protein; GRAM domain-containing protein 4 (GRAMD4)

Positive Control

Human liver, heart or fetal brain.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human GRAMD4 protein

Cellular Localization

Mitochondrion membrane. Colocalizes with COX4I1.

Species Reactivity

Human. Predicted to react in Rat.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

66kDa

Specificity & Comments

The gene encoding GRAMD4 (GRAM domain-containing protein 4) maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. GRAMD4, also designated death-inducing protein (DIP), is a 578 amino acid mitochondrial membrane protein that acts as an essential mediator of the p53-independent E2F-1 death pathway, which is frequently found to be deregulated in several types of cancers. Overexpression of GRAMD4 results in a strong apoptotic response involving caspase-3 activation and cleavage of poly(ADP-ribose)-polymerase. GRAMD4 is expressed in lung and in primary lung squamous cell carcinoma (LSCC) and shows upregulation in mitochondria by E2F1 after addition of 4-hydroxytamoxifen. This evidence suggests that GRAMD4 may be a potential target for cancer therapies.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Zhang J, et al. Chinese Journal of Cellular and Molecular Immunology. 2013 Feb;29(2):190-193.
  2. John K, et al. Cell Death and Differentiation. 2011 May;18(5):874-886. DOI: 10.1038/cdd.2010.153.

Bioinformatics

  •  Entrez Gene ID: 23151
  •  Gene Symbol: GRAMD4
  •  SwissProt: Q6IC98
  •  Chromosome Location: 22q13.31
  •  Unigene: 475150