Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

GRAMD4 (Transcription Factor) Antibody [PCRP-GRAMD4-1A10]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
23151-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
23151-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
23151-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

The gene encoding GRAMD4 (GRAM domain-containing protein 4) maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. GRAMD4, also designated death-inducing protein (DIP), is a 578 amino acid mitochondrial membrane protein that acts as an essential mediator of the p53-independent E2F-1 death pathway, which is frequently found to be deregulated in several types of cancers. Overexpression of GRAMD4 results in a strong apoptotic response involving caspase-3 activation and cleavage of poly(ADP-ribose)-polymerase. GRAMD4 is expressed in lung and in primary lung squamous cell carcinoma (LSCC) and shows upregulation in mitochondria by E2F1 after addition of 4-hydroxytamoxifen. This evidence suggests that GRAMD4 may be a potential target for cancer therapies.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 /
Cellular Localization
Endoplasmic reticulum membrane, Mitochondrion membrane
Gene Name
Positive Control
heart or fetal brain., Human liver
Immunogen
Recombinant full-length human GRAMD4 protein
Alternate Names
GRAM domain-containing protein 4, Death-inducing protein, Death-inducing protein; GRAM domain-containing protein 4 (GRAMD4)

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-GRAMD4-1A10
Chromosome Location
22q13.31
Mol. Weight of Antigen
66kDa

Functions

  • Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53 (PubMed:15565177). Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53. Inhibits TLR9 response to nucelic acids and regulates TLR9-mediated innate immune response (By similarity).

Key References

  • Zhang J, et al. Chinese Journal of Cellular and Molecular Immunology. 2013 Feb;29(2):190-193.
  • John K, et al. Cell Death and Differentiation. 2011 May;18(5):874-886. DOI: 10.1038/cdd.2010.153.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “GRAMD4 (Transcription Factor) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK