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Home >> Antibodies >> Glutamine Synthetase / GLUL

Glutamine Synthetase / GLUL

Mouse Monoclonal Antibody [Clone GLUL/6604]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human liver stained with Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6604) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Western blot analysis of Y79 cell lysate using Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6604).

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Western blot analysis of human liver tissue using Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6604).

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SDS-PAGE Analysis Purified Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6604). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

cell proliferation-inducing protein 59; GLUL; Glutamate ammonia ligase; GS; PIG43; PIG59; Glutamate decarboxylase; glutamine synthase; Glutamine synthetase; glutamine synthetase I; Proliferation inducing protein 43; GLNA; GLNS

Positive Control

Human brain, stomach or thyroid. A-431 or Y79 cells.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm. Mitochondrion.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

42kDa

Specificity & Comments

Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Min, Q., et al. 2019. β-catenin and yes-associated protein 1 cooperate in hepatoblastoma pathogenesis. Am. J. Pathol. 189: 1091-1104.

Bioinformatics

  •  Entrez Gene ID: 2752
  •  Gene Symbol: GLUL
  •  SwissProt: P15104
  •  Chromosome Location: 1q25.3
  •  Unigene: 518525