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Glutamine Synthetase / GLUL Antibody [GLUL/6600]

In Stock
Catalog Number Formulation Size Price
2752-MSM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2752-MSM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2752-MSM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human stomach stained with Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6600) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human stomach stained with Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6600) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

Western blot analysis of human liver tissue lysate using Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6600).

Western blot analysis of human liver tissue lysate using Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6600).

Western blot analysis of Y79 cell lysate using Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6600).

Western blot analysis of Y79 cell lysate using Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6600).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Cow, Horse, Human
Research Areas
Cardiovascular, Mitochondria Marker, Neuroinflammation
Isotype / Light Chain
IgG2c / Kappa
Cellular Localization
Cell membrane, Cytoplasm, Cytosol, Microsome, Mitochondrion
Gene Name
GLUL
Positive Control
Human brain, stomach or thyroid. A-431 or Y79 cells.
Immunogen
Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)
Alternate Names
Glutamine synthetase, Glutamate, ammonia ligase, Palmitoyltransferase GLUL, cell proliferation-inducing protein 59; GLUL; Glutamate ammonia ligase; GS; PIG43; PIG59; Glutamate decarboxylase; glutamine synthase; Glutamine synthetase; glutamine synthetase I; Proliferation inducing protein 43; GLNA; GLNS

Database Links

Entrez Gene ID
2752
SwissProt
P15104

Additional Information

Clone
GLUL/6600
Chromosome Location
1q25.3
Mol. Weight of Antigen
42kDa

Related Antibodies

View All GLUL Antibodies

Functions

  • Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed:30158707, PubMed:16267323). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed:18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed:30158707). May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed:30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed:26711351).

Key References

  • Min, Q., et al. 2019. β-catenin and yes-associated protein 1 cooperate in hepatoblastoma pathogenesis. Am. J. Pathol. 189: 1091-1104.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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