Formalin-fixed, paraffin-embedded human stomach stained with Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6600) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.
Western blot analysis of human liver tissue lysate using Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6600).
Western blot analysis of Y79 cell lysate using Glutamine Synthetase Mouse Monoclonal Antibody (GLUL/6600).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.