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Home >> Antibodies >> Glucose 6-Phosphate Isomerase

Glucose 6-Phosphate Isomerase

Mouse Monoclonal Antibody [Clone CPTC-GPI-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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SDS-PAGE Analysis Purified GPI Mouse Monoclonal Antibody (CPTC-GPI-1). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence Analysis of human MCF-7 cells labeling GPI with GPI Mouse Monoclonal Antibody (CPTC-GPI-1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GPI Mouse Monoclonal Antibody (CPTC-GPI-1).

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Western Blot Analysis of T47D, PC3, HePG2 cell lysates using GPI Mouse Monoclonal Antibody (CPTC-GPI-1).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Glucose 6-Phosphate Isomerase Monoclonal Antibody (CPTC-GPI-1).

Product Details

Synonyms

AMF; aurocrine motility factor; EC 5.2.1.9; GNPI; Gpi1; hexose monophosphate isomerase; neuroleukin; NLK; oxoisomerase; PGI; PHI; phosphoglucose isomerase; phosphosaccharomutase; SA-36; sperm antigen 36

Positive Control

T47D, PC3, MCF-7, HepG2 and A549 cells. Brain, pancreas or thyroid carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length GPI protein

Cellular Localization

Cytoplasm. Secreted.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Beutler, E., et al. 1997.

Bioinformatics

  •  Entrez Gene ID: 2821
  •  Gene Symbol: GPI
  •  SwissProt: P06744
  •  Chromosome Location: 19q13.11
  •  Unigene: 466471
NBT02257