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Home >> Antibodies >> GLIS family zinc finger 3 (GLIS3) / ZNF515

GLIS family zinc finger 3 (GLIS3) / ZNF515

Mouse Monoclonal Antibody [Clone PCRP-GLIS3-1B11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified GLIS3 Mouse Monoclonal Antibody (PCRP-GLIS3-1B11). Confirmation of Purity and Integrity of Antibody.

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Flow cytometric analysis of PFA-fixed HeLa cells. GLIS3 Mouse Monoclonal Antibody (PCRP-GLIS3-1B11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using GLIS3-Monospecific Mouse Monoclonal Antibody (PCRP-GLIS3-1B11).

Product Details

Synonyms

FLJ38999; FLJ90578; GLI similar 3; GLI SIMILAR PROTEIN 3; GLIS family zinc finger 3; MGC33662; OK/KNS-cl.4; Zinc finger protein 515; Zinc finger protein GLIS3; ZNF515

Positive Control

A549 or U-2 OS cells.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IP         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human GLIS3 protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

84kDa

Specificity & Comments

GLIS3 is a member of the GLI similar zinc finger protein family, and encodes a nuclear protein with five C2H2 type zinc finger domains. It functions as both an activator and repressor of transcription, and is specifically involved in the development of pancreatic beta cells, thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full length nature of only two have been determined.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kim, Y.S., et al. 2003. GLIS3, a novel member of the GLIS subfamily of Kr ppel-like zinc-finger proteins with repressor and activation functions. Nucleic Acids Res. 31: 5513-5525.

Bioinformatics

  •  Entrez Gene ID: 169792
  •  Gene Symbol: GLIS3
  •  SwissProt: Q8NEA6
  •  Chromosome Location: 9p24.2
  •  Unigene: 162125