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Home >> Antibodies >> Geminin / DNA Replication Inhibitor

Geminin / DNA Replication Inhibitor

Mouse Monoclonal Antibody [Clone GMNN/3665]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human testis stained with Geminin Mouse Monoclonal Antibody (GMNN/3665). Inset: PBS instead of primary antibody; secondary only negative control.

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Formalin-fixed, paraffin-embedded human spleen stained with Geminin Mouse Monoclonal Antibody (GMNN/3665). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using Geminin Mouse Monoclonal Antibody (GMNN/3665) followed by goat anti-mouse IgG-CF488 (green).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Geminin / DNA Replication Inhibitor Monoclonal Antibody (GMNN/3665).

Product Details

Synonyms

DNA replication inhibitor; Gem; Geminin DNA replication inhibitor; GMNN; RP3 369A17.3

Positive Control

HEK293, HeLa or MCF-7 cells. Human tonsil, testis or triple-negative breast cancer tissue.

Known Applications & Suggested Dilutions

IF, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined

Immunogen

Recombinant fragment of human GMNN protein (exact sequence is proprietary)

Cellular Localization

Nuclear expression in highly proliferating cells.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

35kDa

Specificity & Comments

Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Nishihara, K, et al. Oncology Reports., U.S. National Library of Medicine, 1 May 2009.

Bioinformatics

  •  Entrez Gene ID: 51053
  •  Gene Symbol: GMNN
  •  SwissProt: O75496
  •  Chromosome Location: 6p22.3
  •  Unigene: 234896