SDS-PAGE Analysis Purified Estrogen Receptor beta2 Mouse Monoclonal Antibody (57/3). Confirmation of Integrity and Purity of Antibody.
Formalin-fixed, paraffin-embedded human testis stained with ER-beta2 Mouse Monoclonal Antibody (57/3).
Flow Cytometric Analysis of PFA-fixed MCF-7 cells using ER-beta2 Mouse Monoclonal Antibody (57/3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
This MAb recognizes the estrogen receptor beta 2 isoform, expressed in cell nuclei of a range of human tissues including ovary, placenta, testis and vas deferens. No cross-reactivity is seen with estrogen receptor beta 1 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.