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Home >> Antibodies >> EMI1 (Early Mitotic Inhibitor-1)

EMI1 (Early Mitotic Inhibitor-1)

Mouse Monoclonal Antibody [Clone EMI1/1176]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western Blot of HeLa, HepG2, HEK293 and K562 cell lysates with EMI1 Mouse Monoclonal Antibody (EMI1/1176).

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Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMI1 Mouse Monoclonal Antibody (EMI1/1176).

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Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMI1 Mouse Monoclonal Antibody (EMI1/1176).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using EMI1 Mouse Monoclonal Antibody (EMI1/1176).

Product Details

Synonyms

Early Mitotic Inhibitor-1 (EMI1); FBX5; Fbxo31

Positive Control

HeLa, 293 or HepG2 cells. Ovarian carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml for 60 minutes at RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

56kDa

Specificity & Comments

It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Reimann, J.D., et al. 2001. Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Cell 105: 645-655.

Bioinformatics

  •  Entrez Gene ID: 26271
  •  Gene Symbol: FBXO5
  •  SwissProt: Q9UKT4
  •  Chromosome Location: 6q25.2
  •  Unigene: 520506
NBT01176