2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> EIF4A2 (Eukaryotic Initiation Factor 4A-II)

EIF4A2 (Eukaryotic Initiation Factor 4A-II)

Mouse Monoclonal Antibody [Clone PCRP-EIF4A2-2B5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

SDS-PAGE Analysis Purified EIF4A2 Mouse Monoclonal Antibody (PCRP-EIF4A2-2B5). Confirmation of Purity and Integrity of Antibody.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using EIF4A2 Mouse Monoclonal Antibody (PCRP-EIF4A2-2B5).

Product Details

Synonyms

ATP dependent RNA helicase eIF4A 2; BM-010; DDX2B; EIF4F; eukaryotic translation initiation factor 4A isoform 2; N-terminally processed

Positive Control

HeLa cells.

Known Applications & Suggested Dilutions

ELISA         More Details

ELISA (For coating, order Ab without BSA)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human EIF4A2 protein

Cellular Localization

Nucleus.

Species Reactivity

Human. Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.

Host / Ig Isotype

Mouse / IgG2a

Mol. Weight of Antigen

46kDa

Specificity & Comments

Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a5'-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5' cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Zhang, T., et al. The Journal of Biological Chemistry 288:6166-6177 (2013).
  2. Nucleic Acids Res. 38:D142-D148 (2010).

Bioinformatics