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EIF4A2 (Eukaryotic Initiation Factor 4A-II) Antibody [PCRP-EIF4A2-2B5]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
1974-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1974-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1974-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Summary

Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a5'-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5' cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2a /
Gene Name
Positive Control
HeLa cells.
Immunogen
Recombinant full-length human EIF4A2 protein
Alternate Names
Eukaryotic initiation factor 4A-II, ATP-dependent RNA helicase eIF4A-2, ATP dependent RNA helicase eIF4A 2; BM-010; DDX2B; EIF4F; eukaryotic translation initiation factor 4A isoform 2; N-terminally processed

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-EIF4A2-2B5
Chromosome Location
3q27.3
Mol. Weight of Antigen
46kDa

Functions

  • ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Key References

  • Zhang, T., et al. The Journal of Biological Chemistry 288:6166-6177 (2013).
  • Nucleic Acids Res. 38:D142-D148 (2010).

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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