2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> EIF2S1 / EIF-2 alpha (EIF2A)

EIF2S1 / EIF-2 alpha (EIF2A)

Mouse Monoclonal Antibody [Clone PCRP-EIF2S1-1E2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Immunofluorescence Analysis of PFA-fixed MCF-7 cells using EIF2S1 Mouse Monoclonal Antibody (PCRP-EIF2S1-1E2) followed by goat anti-mouse IgG-CF488 (green).

img

Immunofluorescence Analysis of PFA-fixed HeLa cells using EIF2S1 Mouse Monoclonal Antibody (PCRP-EIF2S1-1E2) followed by goat anti-mouse IgG-CF488 (green). Counterstain is phalloidin.

img

Flow cytometric analysis of PFA-fixed HeLa cells. EIF2S1 Mouse Monoclonal Antibody (PCRP-EIF2S1-1E2) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).

img

SDS-PAGE Analysis Purified EIF2S1 Mouse Monoclonal Antibody (PCRP-EIF2S1-1E2) Confirmation of Purity and Integrity of Antibody.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using EIF2S1 Mouse Monoclonal Antibody (PCRP-EIF2S1-1E2).

Product Details

Synonyms

Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa (EIF2S1); EIF-2alpha, EIF2, EIF2A

Positive Control

HeLa or MCF7 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human EIF2S1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human. Predicted to work in Rat and Zebrafish.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

36kDa

Specificity & Comments

The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2a, eIF2band eIF2g (eukaryotic translation initiation factor 2 a, band g, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2a, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2a controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2a is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S., et al. Nature methods 15.5 (2018 May): 330-338.
  2. Sambucetti, L.C., et al. 1986. Science 234: 1417-1419.

Bioinformatics

  •  Entrez Gene ID: 1965
  •  Gene Symbol: EIF2S1
  •  SwissProt: P05198
  •  Chromosome Location: 14q23.3
  •  Unigene: 151777