Flow cytometric analysis of PFA-fixed HeLa cells. EIF2S1 Mouse Monoclonal Antibody (PCRP-EIF2S1-1C11) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using EIF2S1 Mouse Monoclonal Antibody (PCRP-EIF2S1-1C11).
SDS-PAGE Analysis Purified EIF2S1 Mouse Monoclonal Antibody (PCRP-EIF2S1-1C11). Confirmation of Purity and Integrity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2a, eIF2band eIF2g (eukaryotic translation initiation factor 2 a, band g, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2a, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2a controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2a is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.