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Home >> Antibodies >> E4F1

E4F1

Mouse Monoclonal Antibody [Clone PCRP-E4F1-2D1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence Analysis of PFA-fixed MCF7 cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti-mouse IgG-CF488 (green); counterstain phalloidin (red).

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SDS-PAGE Analysis Purified E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1).

Product Details

Synonyms

E4F transcription factor 1, isoform CRA_b; P120E4F transcription factor; p50E4F; Putative E3 ubiquitin-protein ligase E4F1; RING type E3 ubiquitin transferase E4F1; Transcription factor E4F1

Positive Control

HeLa, Jurkat or MCF7 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human E4F1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

83kDa

Specificity & Comments

E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control. Additionally, E4F1 is thought to function as a ubiquitin ligase that mediates the ubiquitination (and subsequent degradation) of target proteins and may be involved in the p53 tumor suppressor pathway. E4F1, which may be post-translationally phosphorylated or sumoylated, is subject to proteolytic cleavage which results in the creation of a short peptide with specific DNA binding capabilities.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Fernandes, E.R. and Rooney, R.J. 1997. Mol. Cell. Biol. 17: 1890-1903.
  2. Sandy, P., et al. 2000. Oncogene 19: 188-199.

Bioinformatics

  •  Entrez Gene ID: 1877
  •  Gene Symbol: E4F1
  •  SwissProt: Q66K89
  •  Chromosome Location: 16p13.3
  •  Unigene: 513268