E4F1 [PCRP-E4F1-2D1]

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Summary

E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control. Additionally, E4F1 is thought to function as a ubiquitin ligase that mediates the ubiquitination (and subsequent degradation) of target proteins and may be involved in the p53 tumor suppressor pathway. E4F1, which may be post-translationally phosphorylated or sumoylated, is subject to proteolytic cleavage which results in the creation of a short peptide with specific DNA binding capabilities.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Cytoplasm, Nucleoplasm, Nucleus
Gene Name
Positive Control
HeLa, Jurkat or MCF7 cells.
Immunogen
Recombinant full-length human E4F1 protein
Alternate Names
Transcription factor E4F1, E4F transcription factor 1, Putative E3 ubiquitin-protein ligase E4F1, RING-type E3 ubiquitin transferase E4F1, Transcription factor E4F, p120E4F, p50E4F, E4F transcription factor 1, isoform CRA_b; P120E4F transcription factor; p50E4F; Putative E3 ubiquitin-protein ligase E4F1; RING type E3 ubiquitin transferase E4F1; Transcription factor E4F1

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
PCRP-E4F1-2D1
Human Gene Symbol
E4F1
Chromosome Location
16p13.3
Mol. Weight of Antigen
83kDa

Functions

  • May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription.
  • Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes.

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • ,Immunofluorescence (1-2ug/ml)
  • ,Optimal dilution for a specific application should be determined.

Key References

  • Fernandes, E.R. and Rooney, R.J. 1997. Mol. Cell. Biol. 17: 1890-1903.
  • Sandy, P., et al. 2000. Oncogene 19: 188-199.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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